|
KMID : 0380620190510040336
|
|
Korean Journal of Food Science and Technology
2019 Volume.51 No. 4 p.336 ~ p.342
|
|
|
Roles of sugar chains in immunostimulatory activity of the polysaccharide isolated from Angelica gigas
|
|
Shin Kwang-Soon
|
|
|
Abstract
|
|
|
|
To elucidate structure-function relationship of polysaccharide from Angelica gigas, the AGE-2c-I was purified by two successive chromatography steps. AGE-2c-I showed a potent anti-complementary activity in a dose-dependent manner. AGE-2c-I with a molecular weight of 140 kDa comprised four monosaccharides and 13 glycosyl linkages, and strongly reacted with β-glucosyl Yariv reagent. For the fine structure analysis of AGE-2c-I, it was sequentially digested by exo-arabinofuranosidase and endo-galactanase. The results indicated that AGE-2c-I was a typical RG-I polysaccharide with side chains such as highly branched α-arabinan, β-(1→4)-galactan and arabino-β-3,6-galactan. To characterize the active moiety of AGE-2c-I, the anti-complementary activities of AGE-2c-I and its subfractions were assayed. It was observed that the anti-complementary activity of AGE-2c-I was due to the entire structure that resembled RG-I. In addition, arabino-β-3,6-galactan side chain (GN-I) in AGE-2c-I probably plays a crucial role in the anti-complementary activity, whereas α-arabinan side chain (AFN-I) consisting of 5-linked Araf and 3,5-branched Araf partially contributes to the activity.
|
|
|
KEYWORD
|
|
|
Angelica gigas, polysaccharide, rhamnogalacturonan-I, anti-complementary activity, structure-function relationship
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|